24. Huang R*. Methyl-TROSY NMR Spectroscopy in the Investigation of Allosteric Cooperativity in Large Biomolecular Complexes (Book chapter). NMR Spectroscopy for Probing Functional Dynamics at Biological Interfaces. 2022, ISBN 978-1-83916-209-1, Royal Society of Chemistry.


23. Conicella AE*, Huang R*, Ripstein ZA, Nguyen A, Wang E, Löhr T, Schuck P, Vendruscolo M, Rubinstein JL, Kay LE. An intrinsically disordered motif regulates the interaction between the p47 adaptor and the p97 AAA+ ATPase. Proc Natl Acad Sci U S A. 2020 Oct; 117(42):26226-26236. (Joint corresponding author)

22. Huang R*, Ripstein ZA*, Rubinstein JL, Kay LE. Probing cooperativity of N-terminal domain orientations in the p97 molecular machine: synergy between NMR and cryo-EM. Angew Chem Int Ed Engl. 2020 Aug; 59(50): 22423-22426. (Joint corresponding author)

21. Rennella E, Huang R,  Yu Z, Kay LE. Exploring long-range cooperativity in the 20S proteasome core particle from T. acidophilum using methyl-TROSY based NMR spectroscopy. Proc Natl Acad Sci U S A. 2020 Mar; 117(10):5298-5309.

20. Yuwen T, Huang R, Vallurupalli P, Kay LE. A Methyl‐TROSY‐Based 1H Relaxation Dispersion Experiment for Studies of Conformational Exchange in High Molecular Weight Proteins. Angew Chem Int Ed Engl. 2019 May; 58(19):6250-6254.

19. Huang R, Ripstein ZA, Rubinstein JL, Kay LE. Cooperative subunit dynamics modulate p97 function. Proc Natl Acad Sci U S A. 2019 Jan 2;116(1):158-167. (Joint corresponding author)

18. Zhou Y, Damasceno PF, Somashekar BS, Engel M, Tian F, Zhu J,Huang R, Johnson K, Mclntyre C, Sun K, Yang M, Green PF, Ramamoorthy A, Glotzer SC and Kotov N. Unusual multiscale mechanics of biomimetic nanoparticle hydrogels. Nat Commun. 2018 Jan 12;9(1):181.

17. Huang R, Pérez F, Kay LE. Probing the cooperativity of Thermoplasma acidophilum proteasome core particle gating by NMR spectroscopy. Proc Natl Acad Sci U S A. 2017 Nov 14;114(46):E9846-E9854.

16. Brady JP, Farber PJ, Sekhar A, Lin YH, Huang R, Bah A, Nott TJ, Chan HS, Baldwin AJ, Forman-Kay JD, Kay LE. Structural and hydrodynamic properties of an intrinsically disordered region of a germ cell-specific protein on phase separation. Proc Natl Acad Sci U S A. 2017 Sep 26;114(39):E8194-E8203.

15. Huang R, Brady JP, Sekhar A, Yuwen T, Kay LE. An enhanced sensitivity methyl 1H triple-quantum pulse scheme for measuring diffusion constants of macromolecules. J Biomol NMR. 2017 Aug;68(4):249-255.

14. Yuwen T, Huang R, Kay LE. Probing slow timescale dynamics in proteins using methyl 1H CEST. J Biomol NMR 2017 Jul;68(3):215-224.

13. Ripstein ZA, Huang R, Augustyniak R, Kay LE, Rubinstein JL. Structure of a AAA+ unfoldase in the process of unfolding substrate. Elife 2017 6: e25754.

12. Huang R*, Ripstein ZA*, Augustyniak R, Lazniewski M, Ginalski K, Kay LE, Rubinstein JL. Unfolding the mechanism of the AAA+ unfoldase VAT by a combined cryo-EM, solution NMR study. Proc Natl Acad Sci U S A. 2016 Jul 19;113(29):E4190-E4199.

11. Zhang M, Huang R, Ackermann R, Im SC, Waskell L, Schwendeman A, Ramamoorthy A. Reconstitution of the Cytb5-CytP450 Complex in Nanodiscs for Structural Studies using NMR Spectroscopy. Angew Chem Int Ed Engl. 2016 Mar 24;55(14):4497-4499.

10. Rennella E, Huang R, Velyvis A, Kay LE. 13CHD2-CEST NMR Spectroscopy Provides an Avenue for Studies of Conformational Exchange in High Molecular Weight Proteins. J Biomol NMR. 2015 Oct;63(2):187-199.

9. Huang R, Zhang M, Rwere F, Waskell L, Ramamoorthy A. Kinetic and Structural Characterization of the Interaction between the FMN Binding Domain of Cytochrome P450 Reductase and Cytochrome c. J Biol Chem. 2015 Feb 20;290(8):4843-4855.

8. Zhang M, Huang R, Im SC, Waskell L, Ramamoorthy A. Effects of Membrane Mimetics on Cytochrome P450-cytochrome b5 Interactions Characterized by NMR Spectroscopy. J Biol Chem. 2015 May 15;290(20):12705-12718.

7. Zhang M, Le Clair SV, Huang R, Ahuja S, Im SC, Waskell L, Ramamoorthy A. Insights into the Role of Substrates on the Interaction between Cytochrome b5 and Cytochrome P450 2B4 by NMR. Sci Rep. 2015 Feb 17;5:8392.

6. Huang R, Yamamoto K, Zhang M, Popovych N, Waskell L, Ramamoorthy A. Probing the Transmembrane Structure and Dynamics of Microsomal Cytochrome P450 Reductase by Solid-state NMR. Biophys J. 2014 May 20;106(10):2126-2133.

5. Pandey M, Vivekanandan S, Ahuja S, Huang R, Im SC, Waskell L, Ramamoorthy A. Cytochrome-P450-Cytochrome-b5 Interaction in a Membrane Environment Changes 15N Chemical Shift Anisotropy Tensors. J Phys Chem B. 2013 Nov 7;117(44):13851-13860.

4. Ahuja S, Jahr N, Im SC, Vivekanandan S, Popovych N, Le Clair S, Huang R, Soong R, Xu J, Yamamoto K, Nanga R, Waskell L, Ramamoorthy A. A Model of the Membrane-Bound Cytochrome b5-cytochrome P450 Complex from NMR and Mutagenesis Data. J Biol Chem. 2013 Jul 26;288(30):22080-22095.

3. Huang R*, Vivekanandan S*, Brander J*, Abe Y, Naito A, Ramamoorthy A. NMR Characterization of Monomer and Oligomeric Conformations of Human Calcitonin and its Interaction with EGCG. J Mol Biol. 2012 Feb 10;416(1):108-120.

2. Sovadinova I, Palermo E, Huang R, Thoma L, Kuroda K. Mechanism of Polymer-Induced Hemolysis: Nanosized Pore Formation and Osmotic Lysis. Biomacromolecules. 2011 Jan 10;12(1):260-268.

1. Thennarasu S, Huang R, Lee D, Yang P, Maloy L, Chan Z, Ramamoorth A. Limiting an Antimicrobial Peptide to the Lipid-Water Interface Enhances its Bacterial Membrane Selectivity – A Case Study on MSI-367. Biochemistry. 2010 Dec 21;49(50):10595-10605.

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